Circulating reentrant waves promote maturation of hipsc. The xf real time atp rate assay can be used to characterize the cell linetype of interest in a single assay plate. The following items are included in the xfe24 instrument and controller boxes instrumentcontroller quantity. Ocr and ecar rates are key indicators of mitochondrial respiration and glycolysis and these measurements provide a systemslevel view of cellular metabolic function in cultured cells and exvivo samples. We have 1 seahorse xf96 manual available for free pdf download. The xfp extracellular flux analyzer is built on innovative and proven xf technology for live cellbased assays of energy metabolism. It covers the interface views, connectivity and suggestions. This manual is a guide for the usage and functions of the flux. How to hydrate an agilent seahorse xfe96 sensor cartridge. Lower the sensor cartridge onto the utility plate, submerging the sensors in the water 7. Flow meter fmc fmj for lowviscosity, also slightly soiled nutating disc meter for mobile use with flux drum and container pumps fmc 100 or stationary in plant engineering fmc 250.
Seahorse xfp extracellular flux analyzer user manual. Place the sensor cartridge upside down next to the utility plate. Each person should firmly grasp the base of the unit at opposite ends to one another. Extracellular flux analyzer and prep station brand. Seahorse xf96 installation and operation manual pdf download. Pdf advances in the quantification of mitochondrial. View online or download seahorse xf96 installation and operation manual. Compact and easy to use, the xfp analyzer is designed to perform standard xf assays, and is ideal for pairwise comparisons. View and download seahorse xf96 installation and operation manual online. Within this ring, reentrant waves of action potential. Seahorse xfe96 or xf96 extracellular flux analyzer, it is essential to first characterize a specific cell type with respect to its metabolic activity under basal respiration ocr and extracellular acidification ecar. The xl2 analyzer forms the unique combination of a stateoftheart sound level meter, a comprehensive acoustic analyzer as well as a powerful audio analyzer. Seahorse xfp analyzer user manual 7 overview the agilent xfp analyzer measures the rate of change of dissolved oxygen and ph in the media immediately surrounding living cells cultured in a miniplate. Each measurement cycle mix and measure amounts to one time point and, over time, the kinetic data is plotted.
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